Journal: Diabetes

Article Title: Hsp20-Mediated Activation of Exosome Biogenesis in Cardiomyocytes Improves Cardiac Function and Angiogenesis in Diabetic Mice

doi: 10.2337/db15-1563

Figure Lengend Snippet: Altered exosomal contents by overexpression of Hsp20 in cardiomyocytes could be delivered to endothelial cells and native cardiomyocytes. Representative Western blots (A) and their quantitative results (B) showed that higher levels of Hsp20, survivin, and p-Akt encased in TG-Exo than WT-Exo. C: SOD1 levels were increased in TG-Exo, compared with WT-Exo (n = 4 for A–C). *P < 0.05 vs. WT-Exo. D: Green dye–labeled exosomes were detectable in the cytosol of MCECs after exposure to green dye PKH67-labeled WT- and TG-Exo, respectively. E and F: Exosomal Hsp20, p-Akt, and survivin were effectively transported to endothelial cells, compared with control conditions (n = 4). *P < 0.05 vs. control conditions; #P < 0.05 vs. WT-Exo–treated samples. β-Actin was used as a loading control. EC, endothelial cell.

Article Snippet: Cardiac Endothelial Cell Proliferation, Migration, and Tube Formation Assays A mouse cardiac endothelial cell (MCEC) line was purchased from CELLutions Biosystems.

Techniques: Over Expression, Western Blot, Labeling, Control